Antibody-secreting B cells in HIV infection

Antibody secreting B cells and plasma antibody response to rotavirus vaccination in infants from Kolkata India

Background Assessing immune response after rotavirus vaccination consists in measuring serum or plasma IgA and IgG antibodies, but these assays provide very little information about the mucosal immune response. Thus the development of assays for detection of mucosal immune response following rotavirus vaccination is essential. We evaluate to assess circulating antibody-secreting cells (ASCs) as...

Intestinal Extravasation of IgA Antibody-Secreting Cells

Microfluidic Trapping of Antibody-secreting Cells

The development of antibody-based drugs typically begins with the isolation and screening of thousands of antibody-secreting cells, often requiring 4-6 weeks to identify only 1-10 cell lines of interest. We are developing a novel immunobiosensing device to afford direct and rapid assessment of the antibody production of each of thousands of living cells captured on a single slide. Each cell sha...

Interleukin-21 Drives Proliferation and Differentiation of Porcine Memory B Cells into Antibody Secreting Cells

Immunological prevention of infectious disease, especially viral, is based on antigen-specific long-lived memory B cells. To test for cellular proliferation and differentiation factors in swine, an outbred model for humans, CD21+ B cells were activated in vitro with CD40L and stimulated with purported stimulatory cytokines to characterize functional responses. IL-21 induced a 3-fold expansion i...

TLR9 Ligand (CpG Oligodeoxynucleotide) Induces CLL B-Cells to Differentiate into CD20+ Antibody-Secreting Cells

B-cell chronic lymphocytic leukemia (CLL) is the most frequent adult leukemia in the Western world. It is a heterogeneous disease characterized by clonal proliferation and the accumulation of CD5(+) mature B lymphocytes. However, the normal counterpart from which the latter cells arise has not yet been identified. CD27 expression and gene expression profiling data suggest that CLL cells are rel...